Coding

Part:BBa_K4367004:Design

Designed by: August Qvist   Group: iGEM22_Chalmers-Gothenburg   (2022-08-01)


vioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 172
    Illegal AgeI site found at 484
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for Saccharomyces cerevisiae. Overhangs for making the part moClo compatible as a type 3 moClo part.


Source

This part has been codon optimized for saccharomyces cerevisiae and ordered as synthetic DNA. The original gene is found in the Chromobacterium violaceum genome. DNA sequence converted from peptide sequence found through Genbank: https://www.ncbi.nlm.nih.gov/protein/QRQ17046.1

References